scATAC integration

Question

[bioinformatics.girl]

I am integrating 5 different datasets for a scATAC-seq analysis.

I have had the following error persist for weeks; have looked online as well for possible solutions yet nothing has worked. Has anyone run into this issue?

The following code is:

object.list = list(sample1,sample2, sample3, sample4, sample5)

integration.anchors <- FindIntegrationAnchors(
  object.list = object.list,
  anchor.features = rownames(sample1),
  reduction = "rlsi",
  dims = 2:30
)


library(future)

plan("sequential")

options(future.globals.maxSize = 16000 * 1024^2)

integrated<-IntegrateData(
  integration.anchors, 
  new.assay.name = "integrated",
  normalization.method = c("LogNormalize", "SCT"),
  dims = 1:27, 
  sd.weight = 1,
  eps = 0, 
  verbose = TRUE 
)