Line chart plotting

Question

[Adyasha]

Hi everyone,

I have a dataset like this . A, B, C, D, E, and F are different samples. I want to create a line chart that has a baseline for all these values and then sample specific fluctuations basing the baseline.

DATE    A   B   C   D   E   F
10-02-2023  15700   0   0   0   0   0
14-02-2023  36800   0   0   0   0   0
17-02-2023  23,700.00   23000   0   0   0   0
21-02-2023  0   8400    0   0   0   0
24-02-2023  0   8000    0   0   0   0
10-03-2023  0   0   831100  0   0   0
14-03-2023  0   0   72000   0   0   0
22-03-2023  0   565300  0   0   0   0
30-03-2023  0   865000  0   1618300 0.28    0
04-04-2023  0   58.466  0.307   19.268  0   0
10-04-2023  0   114.558 0   9.801   0   0
11-04-2023  0   0   1.911   0   0   0
12-04-2023  0   0   0   0   0   0.194

I took 8400 as median for all entries for samples . so 8400 will be the baseline, then how can I plot these samples in different colours?

or just a baseline and one plot showing fluctuation upper and lower to the baseline also works .

for PCR ct value calculation .

I am new to this. Please help

I want a plot like this.

[rpolicastro]

You need to pivot your data from wide to long using pivot_wider. You can then plot it using ggplot similar to the suggestions given in your previous posts.