Entering edit mode
12 months ago
yojetsharma
•
0
Are we required to carry out ddPCR to validate the DGEs obtained from scRNA seq analyses?
Are we required to carry out ddPCR to validate the DGEs obtained from scRNA seq analyses?
There is no standard if you ask me. I cannot recall a single-cell paper doing qPCR. If you want to validate markers then rather do immuno/protein confirmation or FACS. For qPCR would need to isolate cells first via FACS anyway so why bother. It's imo an overrated assay with many drawbacks and little power.
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You could do in situ expression analysis if any of your cell types are available via histology. Sometimes people make GFP or mCherry reporter constructs to show expression in a given cell type. It sort of depends on what tools are available for your system and how much you want to say about your results.