desgin a primer for CDS
0
0
Entering edit mode
19 months ago
yuxiang • 0

I want to desgin a primer for the mRNA, now I have some cell samples, it contains gDNA, mRNA(not only the target gene) More detail: I need to use the pcr to amplyfy the target gene mRNA(mature), now I need to design a primer which can produce the CDS( mRNA).

gene pcr • 962 views
ADD COMMENT
0
Entering edit mode

what is the question ? are you looking for a software ? good practices ?

ADD REPLY
0
Entering edit mode

sorry for my late reply, I am a student, my profossor ask me to dsgin a primer for the specific cDNA, I have solved now, thanks for your reply.

ADD REPLY
0
Entering edit mode
ADD REPLY
0
Entering edit mode

how can you know the primer is not bind to other gene site? how can you desgin an experiment? I use the FISH to identify the gene, how about you?

ADD REPLY

Login before adding your answer.

Traffic: 2438 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6