Prefecth-orig and fasterq-dump not working when downloading SRA files (v3.0.5)
1
0
Entering edit mode
2.4 years ago
cpaguay • 0

Hi everyone!

I am having some problems when I try to download SRA files. Yesterday I was trying to download a set of SRA files using SRA Toolkit 3.0.2 and it didn't work. I thought that it was a problem with the version, so I just installed the new one (3.0.5) (I am using Windows and CMD), I used vdb-config -i to install it, and then try the following command and appeared this:

prefetch-orig.exe" SRR9202382

2023-05-09T18:15:09 prefetch-orig.3.0.5 sys: encryption failed while validating token within cryptographic module - Verification issue 0x4008 for this certificate: ( cert. version : 3 serial number : 00 issuer name : C=US, O=The Go Daddy Group, Inc., OU=Go Daddy Class 2 Certification Authority subject name : C=US, O=The Go Daddy Group, Inc., OU=Go Daddy Class 2 Certification Authority issued on : 2004-06-29 17:06:20 expires on : 2034-06-29 17:06:20 signed using : RSA with SHA1 RSA key size : 2048 bits basic constraints : CA=true )
2023-05-09T18:15:09 prefetch-orig.3.0.5 sys: mbedtls_ssl_get_verify_result for 'locate.ncbi.nlm.nih.gov' returned 0x4008 ( !! The certificate is not correctly signed by the trusted CA !! The certificate is signed with an unacceptable hash. )
2023-05-09T18:15:09 prefetch-orig.3.0.5: Current preference is set to retrieve SRA Normalized Format files with full base quality scores.

Tried the same with fasterq-dump and showed this

fasterq-dump.exe" SRR9202382

2023-05-09T18:18:16 fasterq-dump.3.0.5 sys: encryption failed while validating token within cryptographic module - Verification issue 0x4008 for this certificate: ( cert. version : 3 serial number : 00 issuer name : C=US, O=The Go Daddy Group, Inc., OU=Go Daddy Class 2 Certification Authority subject name : C=US, O=The Go Daddy Group, Inc., OU=Go Daddy Class 2 Certification Authority issued on : 2004-06-29 17:06:20 expires on : 2034-06-29 17:06:20 signed using : RSA with SHA1 RSA key size : 2048 bits basic constraints : CA=true )
2023-05-09T18:18:16 fasterq-dump.3.0.5 sys: mbedtls_ssl_get_verify_result for 'locate.ncbi.nlm.nih.gov' returned 0x4008 ( !! The certificate is not correctly signed by the trusted CA !! The certificate is signed with an unacceptable hash. )
Failed to call external services.

Does anyone have an idea of what is going on?

Windows SRAtoolkit sra • 1.6k views
ADD COMMENT
1
Entering edit mode
2.4 years ago
GenoMax 154k

Since you are using the latest version (which was suggested as a solution for a previous thread) it does not seem to be working for you.

Do yourself a favor and download using direct URL's.

https://ftp.sra.ebi.ac.uk/vol1/fastq/SRR920/002/SRR9202382/SRR9202382_1.fastq.gz
https://ftp.sra.ebi.ac.uk/vol1/fastq/SRR920/002/SRR9202382/SRR9202382_2.fastq.gz

These URL's were obtained using: https://sra-explorer.info/

ADD COMMENT

Login before adding your answer.

Traffic: 2528 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6