Finding Enhancers using Genomation library

Question

[Pankaj]

Hi everyone,

Greetings!

I am able to find CpG islands and shores, but now i want to find enhancers. please help me find a way to get information about enhancers using genomation library.

Thanks, and best regards!

Answer 1

[ATpoint]

You cannot derive enhancers from methylation profiles. Enhancers are regulatory elements, and they either are methylated or not. There is literature showing both depending on the transcription factors that bind there. Methylation is an epigenetic modification, and genomation is a framework to analyse it, but there is no direct approach to derive enhancers solely from methylation profiles. You would at least need chromatin accessability data or ChIP-seq for enhancer-associated histone marks such as H3K27ac for this.