Entering edit mode
2.2 years ago
htrcek
•
0
Dear community,
I sequenced transcriptome (cDNA-PCR Sequencing Kit) using Nanopore long-read sequencing technology (flow-cell R9.4.1).
I have a question regarding a low Quality score (below 9), which caused that 3.84 Gb were classified as "FAIL", and only 1.68 Gb as "PASS". Since we are only optimizing the protocol, the first sequencing was done on an expired flow-cell. However, translocation speed was sufficient throughout the experiment.
Can be low Q score a result of the expired flow-cell or are there any other explanations for this?
Thank you in advance!
Kind regards.
Have you checked the "failed" sequences by aligning them if you have a reference available? Those may still be usable.