Entering edit mode
8 months ago
abmmki
•
0
Want to run monocle2 for a single cell RNAseq data processed using Seurat, but encountering following problem.
library(monocle)
Seurat An object of class Seurat 41445 features across 55683 samples within 1 assay Active assay: RNA (41445 features, 1850 variable features) 4 dimensional reductions calculated: pca, tsne, umap, harmony
cds <- importCDS(Seurat, import_all=TRUE)
cds <- estimateSizeFactors(cds)
cds <- estimateDispersions(cds)
Error in h(simpleError(msg, call)) : error in evaluating the argument 'x' in selecting a method for function 'rowMeans': unable to coerce from TsparseMatrix to [CR]sparseMatrixwhen length of 'i' slot exceeds 2^31-1
How to solve this?
Thanks.