Error in h(simpleError(msg, call)) in monocle2
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Entering edit mode
8 months ago
abmmki • 0

Want to run monocle2 for a single cell RNAseq data processed using Seurat, but encountering following problem.

library(monocle)

Seurat An object of class Seurat 41445 features across 55683 samples within 1 assay Active assay: RNA (41445 features, 1850 variable features) 4 dimensional reductions calculated: pca, tsne, umap, harmony

cds <- importCDS(Seurat, import_all=TRUE)

cds <- estimateSizeFactors(cds)

cds <- estimateDispersions(cds)

Error in h(simpleError(msg, call)) : error in evaluating the argument 'x' in selecting a method for function 'rowMeans': unable to coerce from TsparseMatrix to [CR]sparseMatrixwhen length of 'i' slot exceeds 2^31-1

How to solve this?

Thanks.

pseudotime scRNAseq monocle2 • 449 views
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