Why figure from guppy basecaller show only 1 base in each event?
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20 months ago

Hi, I am a beginner in nanopore. So I study about the guppy basecaller and try to use it. In normally from each peak in raw signal that represent current for each 5-mer. I wonder why in this picture that show the only 1 base for each peak instead show AACAT, CCGGT?

enter image description here

basecally guppy nanopore • 595 views
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Probably because they wanted to keep the representation simple and are just showing the consensus calls on one strand.

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7 months ago
Leon ▴ 130

briefly,it use middle base to represent 5mer, similarly in tombo resquiggle

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