ONT long reads and IGV
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4 months ago
Kay • 0

I was wondering how IGV renders and outputs a visualization of ONT long reads that have possible CNVs. How do I determine the breakpoint of the CNVs from this visualization? Also for background, I realize there are a lot of soft clipped bases to the left and to the right of the detected CNV (found due to read depth). Do the soft-clipped reads indicate the breakpoints of the amplicon? If so do these soft clipped bases align to the amplicon?

I ask all these questions because we were under the assumption the reads would wrap unto themselves around the amplicon, which led to increased coverage.

long-reads IGV ONT • 207 views
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