Entering edit mode
8 months ago
optimistsso4co3
▴
120
Hello everyone,
I'm reaching out to inquire if anyone is aware of a protocol or publication that delineates a methodology for integrating Illumina whole genome sequencing (WGS) data with long-read sequencing data from Nanopore technologies. Is there a recommended approach for this type of data fusion in the context of bioinformatics analysis?
Please add more details, no one can help you without more info.
Hard to say if this fits, but you could make a consensus call among the two methodologies using a tool like SURVIVOR. However, this would be dangerous as long and short read data have different sensitivities to detect different classes of SV, so you may end up with a worse overall dataset by merging different sequencing techniques.