Hello, I am working with two sample conditions: control vs treatment.

My sample space looks like this:

ENA Number File Name condition

SRX4455233 E_C_1 control

SRX4455236 E_A_1 treatment

When i tried running DESeq2 on this, the error pops up:

dds <- DESeq(dds)

estimating size factors estimating dispersions Error in checkForExperimentalReplicates(object, modelMatrix) :

The design matrix has the same number of samples and coefficients to fit, so estimation of dispersion is not possible. Treating samples as replicates was deprecated in v1.20 and no longer supported since v1.22.

What does this mean? How can I work on this? I cannot perform DESeq2 on lesser sample space?

Without replication, we cannot perform DE analysis deseq2. Try edgeR

No, the idea is to use replication.