DADA2 truncLen value
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5 weeks ago
Bioinfo ▴ 20

Hello i have done illumina sequencing and the median of my forward reads is 281 and reverse is 277 , i have plot barplot of reads length in attached pictures

I want to know how can i choose truncLen and what is the optimal truncLen values to use based on these reads lengths in forward and reverse files. Thank you

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metabarcoding phyloseq DADA2 illumina • 160 views
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