Hello,
I am working on deriving HRD scores for human geome samples.
As part of this, the quality report after adaptor triming showsa broad range in the reads length distribution. I am attaching the image here.
Do I require to filter the reads of specific length before proceeding for alignmnet with reference (or) is it good to proceed for alignmnet as is? I could see there is an intense length distribution below 150 bp and majority of the reads are around 150 bp. What I suspect is to filter the reads below 150 bp?
Please anyone give your thoughts.
Your inputs are greatly appreciated.
This kind of statement does not help. On a forum powered by volunteers' time, pushing your sense of urgency has an effect of throwing people off as people might move on to something they can actually help with instead of dealing with more pressure during their time off their actual jobs.