fmols for nanopore library loading

Question

[njornet]

We use the LSK114 kit and in the protocol, when loading a library it asks for a specific amount of fmol which requires knowing the length of the DNA fragments. Is there a way to estimate this without having to analyse the sample? If not, is it better to over feed it?

Answer 1

[GenoMax]

While this is not a bioinformatics question per se, you can use tapestation or a similar protocol to estimate fragment sizes.

[njornet]

Oh yeah, you're right I meant to ask in the nanopore community. My bad!