Entering edit mode
13 days ago
mathavanbioinfo
▴
90
Hi there I need suggestions and guidance. I am working on a meta-transcriptome project focusing on environmental samples, primarily bacteria. The Trinity assembler was used to obtain the assembly. I filtered a list of transcripts for primer design, but most of the transcripts do not start with start or stop codons. What factors should I consider for designing primers for those transcripts without start and stop codons?