The majority of the sequences in Trinity ID do not start from start codons. Can the sequences still be used for primer design and validation?
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5 weeks ago

Hi there I need suggestions and guidance. I am working on a meta-transcriptome project focusing on environmental samples, primarily bacteria. The Trinity assembler was used to obtain the assembly. I filtered a list of transcripts for primer design, but most of the transcripts do not start with start or stop codons. What factors should I consider for designing primers for those transcripts without start and stop codons?

Primers TRINITY • 569 views

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