BEAST2.5 Chloroplast Divergence Time using Fossil Records

Question

[a.bibek52]

Hello Community,

I am trying to use BEAST2.5 for divergence time analysis. I have taken 4 cp genomes of Gynura species and chosen Artemisia as an outgroup (trying to replicate the Molecular clock of https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-019-6196-x#Sec2, however only including 5 cp genomes). I aligned the entire cp genomes using MAFFT and have a NEWICK output. Now, I am not sure what I need to do. Can anyone please help me in this stuff?

Thank you.

[Ram]

Do not use all caps - it's bad etiquette. I've fixed things for you this time.