. I am using mtDNA PacBio data to calculate nucleotide diversity. For my analysis, I have been utilizing bioinformatics tools on a Linux platform. Initially, I tried using Samtools and BCFtools, followed by running a script in Python (PyCharm) to measure nucleotide diversity, but I was unsuccessful. Suggest me some tools for these calculations. I humbly request your guidance from the beginning. Could please someone help me?
Thank you for your assistance.
Best regards,
what does it mean ?
Initially, I tried using Samtools and BCFtools, followed by running a script in Python (PyCharm) to measure nucleotide diversity![nucleotide_diversity_screenshot](/media/images/16181ba8-fa3c-47e4-ba7f-55f9a57d)