Fastqc: kmer-content failed

Question

[Joseph]

Hello everyone, I use paired data (forward and reverse) from illumina and when checking the quality with FASTQC, an error appears in the size of Kmers, but only in the reverse sequence. I eliminated adapters and low quality sequences. Why is there an error in the size of kmers? Could it be because of the type of sequencing?

Thank you very much to all

[GenoMax]

Could it be because of the type of sequencing?

Potentially. You have not told us what kind of experiment this is.

I eliminated adapters and low quality sequences.

Are you completely certain? How many sequences/bases did you lose? Can you show us the plots of R1/R2 for original data.