CRISPR pooled screening analysis: STARS analysis 'between the groups'

Question

[Yummi Lee]

Hi everyone,

I'm working with Negative Binomial Distribution (STARS) analysis, and I'm using the tool available here: https://portals.broadinstitute.org/gpp/public/analysis-tools/crispr-gene-scoring

I've successfully completed the hit calling and sgRNA ranking analysis for individual samples (I have 6 samples per group and 4 groups in total). However, I'm now stuck on how to compare the hit calling and statistical significance for sgRNA enrichment or depletion 'between the groups'.

Could anyone guide me on how to approach this? Any help would be greatly appreciated. Thank you!