Entering edit mode
10 months ago
Alaa Elsaghir
•
0
Hello everyone,
I have a dataset representing mean fluorescence intensity (MFI) measured using a Luminex device. Due to a high volume of samples, I conducted measurements across four plates, each containing control samples.
After applying log base 2 transformation to the data, I calculated the median for all values on each plate and subtracted that median from the respective values. However, I've encountered zero and negative values in my results.
What is the best approach to handle these negative values? Should I add a constant to shift the values, and if so, what constant should I use? Should the constant be applied universally across all plates, or should each plate have its own specific constant?
Thank you for your assistance!
