mageck mle design matrix

Question

[Assa Yeroslaviz]

I have two later time points I would like to compare with time point t0.

I have wo t0 samples and each four t8 and t16 samples. The count matrix is as such:

Would this design matrix be correct, when running the mageck-mle:

sample  baseline    t8  16
ctrl_t0_1   1   0   0
ctrl_t0_2   1   0   0
ctrl_t8_1   1   1   0
ctrl_t8_2   1   1   0
ctrl_t8_3   1   1   0
ctrl_t8_4   1   1   0
ctrl_t16_1  1   0   1
ctrl_t16_2  1   0   1
ctrl_t16_3  1   0   1
ctrl_t16_4  1   0   1

And would the results be similar to running the two comparisons (t8 and t16) separately?

thanks

Assa