no "-" strand in transcriptome alignment

Question

[baibhu1234]

Hello everyone,

I am working with DRS reads and i have performed a transcriptome alignment of the DRS reads.

i can see that there are very few or no alignments on "-" strand and all are on "+" strand.

Am i missing some concept here? please anyone can explain?

Answer 1

[GenoMax]

This was answered in a separate thread: mapping to transcriptome.fasta vs genome.fasta