Entering edit mode
4 weeks ago
Beatrice
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0
Hi everyone,
I have been prepping libraries for low biomass samples and got strange results from my most recent chip. DNA was extracted from oral and nasal swabs, and library prep was performed with a NEB Next Ultra II FS DNA library prep kit. One of my samples appears to inch up on the Y-axis in a way that I have not seen before. link to SeqAnswers post regarding this issue with a photo attached Does anyone have an idea on why this phenomena would occur? I'm aiming for peaks around 300bp, which can be seen in my other samples. The problematic trace comes from an oral swab.
Thanks for your help,
Beatrice
Cross-posted to SeqAnswers: https://www.seqanswers.com/forum/applications-forums/sample-prep-library-generation/326545-issue-with-agilent-2100-bioanalyzer-hs-dna-assay-trace
More appropriate for that forum.