Query Regarding NGS Analysis with TruSeq UMI Adapters

Question

[1769mkc]

TruSeq UMI adapters used for the cffDNA samples as per Twist cfDNA Library

The TruSeq UMI adapters introduce inline UMIs at the start of Read 1/Read 2, I would like to understand what modifications are required in the downstream analysis pipeline.

So the Query Regarding NGS Analysis with TruSeq UMI Adapters are

1. The appropriate method for UMI extraction, grouping reads by UMI, Steps for consensus read generation, before proceeding with variant calling.

Any suggestion would be really helpful

Answer 1

[GenoMax]

Twist provides a guide to process the data containing UMI: https://www.twistbioscience.com/sites/default/files/resources/2023-03/DOC-001337_TechNote-ProcessingSequencingDataUtilizingUMI-REV1-singles.pdf