Power analysis for miRNA sequecing

Question

[abedkurdi10]

Hello everyone,

I need your recommendations on how to do power analysis for miRNA sequencing.

Thanks!

[ATpoint]

I don't think power analysis is common in sequencing. There are a couple of tools and papers you could try, but they will probably tell you to do more replicates than is feasible and financially affordable. Why don't you tell about the experimental setup and rough idea of the budget, and then we wo forward from there?

[abedkurdi10]

Thank you for your reply!

I still have no idea about the budget and experimental design yet, it was just like short conversation. I will get back to you if I need further details. I got your point for now.

Much appreciated!

[GenoMax]

While miRNA is not specifically covered, there is a good overview of power calculation methods for NGS in this paper --> https://pmc.ncbi.nlm.nih.gov/articles/PMC6291796/

Looks like there is one for scRNAseq --> https://academic.oup.com/bioinformatics/article/36/19/4860/5866544