Whole Genome Sequencing IG regions

Question

[719]

I am looking at some sequencing data in https://doi.org/10.1038/s41588-024-01853-0 and am particularly interested in the loci around the immunoglobulin regions, on Chromosomes 2,14 & 22. They use Illumina HiSeq2000 & HiSeq2500 and a large chunk of somatic mutations found are in or around the immunoglobulin genes, and many not in V genes. Worth noting this is from plasma cells, that will have undergone recombination and somatic hypermutation as these loci. Is this data interesting or did more need to be done to ensure correct mapping of reads etc?

Thanks in advance

[Pierre Lindenbaum]

please define "interesting"

[ATpoint]

The question is what you are after. Is this a disease setup, or...well, what is this all about. Generally, these regions are hotspots of hypermutation, so a lot of varians are expected. Is there a scientific question at hand or are you fishing for a project?

[719]

Sorry for the poor wording of the original post, also should say I really appreciate people taking a moment to look at my post. My main concern is that given there are SHM & recombination in these regions, are the calls I am seeing reliable or should I take them with something of a pinch of salt?