Active Site Of Enzymes
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9.0 years ago

Hi all,

I have a list of UniprotACs corresponding to enzymes (no structure available for most of them). I'd like to identify residues in the active site. For now, I am looking at InterPro and extracting regions with the tag "site". Is this good practice? Any better option?

Many thanks in advance,

Miquel

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If the list is small, you can do homology modeling and look at cavities + key residues. (or) atleast for few you can do a sanity check to see if the regions you had extracted resembles an active site in a model.

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9.0 years ago
Neilfws 49k

In a UniProt record, the key that you are looking for in the feature table is ACT_SITE. For example in P00720 (T4 lysozyme):

FT   ACT_SITE     11     11       Proton donor.
FT   ACT_SITE     20     20       Nucleophile.


So you could grep for that, or parse the record with the parsing library of your choice.