Question: Get Amino Acid Changes From Snp Calls
0
gravatar for rohan
5.2 years ago by
rohan100
United States
rohan100 wrote:

hi.. my aim is to convert snps to amino acid changes..

for calling snps i am using sam2tsv (https://github.com/lindenb/jvarkit/wiki/SAM2Tsv - a great tool by Pierre Lindenbaum ).. after that i want to see amino acid changes owing to codon changes.. for that i am parsing the sam2tsv output using matlab.. but as the matlab is very slow in doing that..

i wonder if there is any tool available.. that gives the information regarding amino acid/ codon changes from SNP calls..

eg. ref (A T G) to seq (G G A)

will give ref(M) to seq(G) ..

may be sam2tsv can be edited to give an option to do so.. :)

amino-acids picard snp • 2.6k views
ADD COMMENTlink modified 5.2 years ago by Gvj440 • written 5.2 years ago by rohan100
2
gravatar for Gvj
5.2 years ago by
Gvj440
Netherlands
Gvj440 wrote:

Hi Rohan, You may try snpeff , its pretty easy to run if you have an annotation of genes.

ADD COMMENTlink written 5.2 years ago by Gvj440

i have done sequencing of a single gene(600nts) carrying different amino acid mutations along the length of the gene.. i want to count the occurrence of mutations as depth per mutation.. how should i make the annotation file in this case? is snpeff applicable for this case?

ADD REPLYlink written 5.2 years ago by rohan100
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