Hello, we're getting RNAseq data from increasing numbers of "emerging" model species for which little is known. The data often wasn't meant to provide population genetic estimates, but could perhaps be used to provide some.
Is it possible to estimate Ne from the SNP patterns that can be identified from:
- a single. sequence pooled from many individuals from a single population?
- a single diploid genome?
- RNAseq data where multiple individual from a single population (siblings or not) were independently sequenced.
A somewhat related question, but specific to pools is here.