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Answer: Answer: Sequence read length shorter than flow cell specification
4 days ago by swbarnes2 14k
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215
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Answer: Answer: from row count to tpm
7 days ago by swbarnes2 14k
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826
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Answer: Answer: Adding CB tag to bam file
11 days ago by swbarnes2 14k
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651
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Answer: Answer: DEG analysis of RNA-seq data across multiple tissues and two conditions
12 days ago by swbarnes2 14k
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574
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Answer: Answer: Removing adapter/primer sequence from reads while keeping flanking regions
28 days ago by swbarnes2 14k
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325
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Answer: Answer: Find out shared differentially expressed genes between two cell lines due to kno
5 weeks ago by swbarnes2 14k
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298
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Answer: Answer: Detecting viruses in human rna seq data
5 weeks ago by swbarnes2 14k
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411
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Answer: Answer: Is it better to pool samples prior to running deseq2, or should the algorithm be
6 weeks ago by swbarnes2 14k
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543
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Answer: Answer: Overriding a color palette in R to emphasize specific data points
6 weeks ago by swbarnes2 14k
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567
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Answer: Answer: RNA_seq
6 weeks ago by swbarnes2 14k
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363
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Answer: Answer: Is it feasible to merge Illumina RNA-Seq data with varying read lengths?
7 weeks ago by swbarnes2 14k
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378
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Answer: Answer: Re. Batch effect correction in data with a design matrix without full rank
7 weeks ago by swbarnes2 14k
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523
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Answer: Answer: RNA Seq Sample Outlier
7 weeks ago by swbarnes2 14k
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397
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Answer: Answer: STAR alignment setting for RNA-Seq data from mouse background expressing a human
7 weeks ago by swbarnes2 14k
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437
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Answer: Answer: (STAR) What causes a high number of reads that are unmapped because they are too
7 weeks ago by swbarnes2 14k
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308
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Answer: Answer: Single cell rna sequencing analysis using R help
7 weeks ago by swbarnes2 14k
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415
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Answer: Answer: single cell rna seq-how to DEG analyze??(full code & Dimplot added)
8 weeks ago by swbarnes2 14k
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247
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Answer: Answer: biological replicate in STAR command
8 weeks ago by swbarnes2 14k
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381
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Answer: Answer: PCA plot analysis
9 weeks ago by swbarnes2 14k
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440
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Answer: Answer: ENA fastq file shown as single-end but has 2 fastq files: X.fastq.gz and Y.fastq
9 weeks ago by swbarnes2 14k
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557
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Answer: Answer: Trouble Understanding DESeq2 Design Formula ("Model Matrix is not Full Rank")
11 weeks ago by swbarnes2 14k
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558
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Answer: Answer: Help interpreting Samtools Flagstat results
3 months ago by swbarnes2 14k
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1
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456
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Answer: Answer: Running Deseq2 with all samples vs samples for each comparison separately
3 months ago by swbarnes2 14k
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321
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Answer: Answer: Interpretation of output from MiXCR TCR sequencing
3 months ago by swbarnes2 14k
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356
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Answer: Answer: Strand information from reverse stranded RNA-seq
4 months ago by swbarnes2 14k
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628
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Answer: Answer: bcl2fastq problems with the yield
4 months ago by swbarnes2 14k
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475
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Answer: Answer: Are 10x cellranger-arc ATAC bam files deduplicated?
5 months ago by swbarnes2 14k
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397
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Answer: Answer: Bulk and Single Cell RNA-seq online course
5 months ago by swbarnes2 14k
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816
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Answer: Answer: STAR ALIGNMENT
5 months ago by swbarnes2 14k
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935
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Answer: Answer: Handling multiple fastq files per sample, per lane, per read out of the Cellrang
5 months ago by swbarnes2 14k
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0
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749
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Answer: Answer: RNAseq how to map Mouse+Virus Genome with STAR
5 months ago by swbarnes2 14k
2
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742
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Answer: Answer: batch correction in DESeq2
6 months ago by swbarnes2 14k
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460
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Answer: Answer: Help with DESeq2 Analysis of Multiple Conditions
6 months ago by swbarnes2 14k
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753
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Answer: Answer: DESeq2: DESeqDataSetFromMatrix Error
6 months ago by swbarnes2 14k
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1
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900
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Answer: Answer: I have performed Trimmomatic run for adapter removal. QC report shows drop in th
7 months ago by swbarnes2 14k
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749
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Answer: Answer: DESeq2 analysis using two featureCounts generated from different studies
7 months ago by swbarnes2 14k
5
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1.1k
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Answer: Answer: Adding a control sample to bulk RNA-seq
7 months ago by swbarnes2 14k
2
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0
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512
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Answer: Answer: DESeq2 design comparing changes through 2 timepoints of 2 different treatments.
7 months ago by swbarnes2 14k
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0
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501
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Answer: Answer: sequencing machines output files layout
7 months ago by swbarnes2 14k
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1
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536
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Answer: Answer: DESeqDataSetFromMatrix error
7 months ago by swbarnes2 14k
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460
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Answer: Answer: Deseq2
7 months ago by swbarnes2 14k
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752
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Answer: Answer: DESEQ2 analysis - PCA plot
8 months ago by swbarnes2 14k
0
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0
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463
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Answer: Answer: Add gene counts of same samples run on two lanes (Novaseq)
9 months ago by swbarnes2 14k
2
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1
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635
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Answer: Answer: bcl2fastq not recognizing multiple barcode-mismatch argument
9 months ago by swbarnes2 14k
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1
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1.1k
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Answer: Answer: Is RSEM required for RNA-seq data analysis using STAR and edgeR?
9 months ago by swbarnes2 14k
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0
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677
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Answer: Answer: 10x genomics hashtag samples
9 months ago by swbarnes2 14k
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1
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621
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Answer: Answer: DESeq2, Time-course interpretation
9 months ago by swbarnes2 14k
4
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0
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1.9k
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Answer: Answer: Multiple testing correction in RNA-Seq
10 months ago by swbarnes2 14k
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0
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2.2k
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Answer: Answer: How to create a Seurat object from the `xxx_metadata.txt.gz ` and `xxx_counts.t
10 months ago by swbarnes2 14k
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1
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1.8k
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Answer: Answer: Getting sequences from fastq file using Grep command
10 months ago by swbarnes2 14k
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