Question: Using ensembl biomart retrieving orthologous codin sequence
0
gravatar for ishengomae
5.5 years ago by
ishengomae100
Tanzania, United Republic Of
ishengomae100 wrote:

I have list of transcript IDs for the cow coding sequences and I want to use these IDs to retrieve orthologous sequences from other ensembl species. One such ID is ENSBTAT00000000080.

With the ensembl BioMart the procedure seemed intuitive to me....
1. Ensembl 75 as database
2. Dataset: Bos taurus
3. Filters: Gene (checked the Id list box and placed list of Ids); Multi-species comparison (checked the Homolog filter box and selected Orthologous genes (e.g Dog Orthologous genes)
4. Attributes: ticked the Sequence button at the top.
5. Click to get results.

....until the results seems not the correct ones! I am getting 100% identical sequences for whatever organism i want orthologs. For example, ENSBTAT00000000080 returns the same sequences in dog as in duck. Something is not right. What step I am missing?

gene • 2.0k views
ADD COMMENTlink modified 5.5 years ago by Vitis2.2k • written 5.5 years ago by ishengomae100
0
gravatar for Devon Ryan
5.5 years ago by
Devon Ryan92k
Freiburg, Germany
Devon Ryan92k wrote:

The sequence is coming from the database you're querying, so it'll always be the same. You need to get a list of orthologous gene/transcript IDs and then query their sequence in the database for that species. Perhaps you can do that in biomart online, but if not it's doable in the biomaRt package in R by just programmatically doing those steps.

ADD COMMENTlink written 5.5 years ago by Devon Ryan92k

Thanks very much.

ADD REPLYlink written 5.5 years ago by ishengomae100
0
gravatar for Vitis
5.5 years ago by
Vitis2.2k
New York
Vitis2.2k wrote:

Take a look at ensembl compara perl API, with which you could query orthologous genes (predicted by ensembl using MCL and gene trees) in batches. 

ADD COMMENTlink written 5.5 years ago by Vitis2.2k
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