Why is the sequence shown in the UCSC genome browser a complement of the BDGP5 sequence (fasta downloads from FlyBase and ensembl)?
The fasta download from FlyBase for a particular region:
>2L:8438269..8438280 species=Dmel; strand='+'; loc=2L:8438269..8438280; CTTTCCAGCACT
The UCSC genome browser displays a sequence as follows (screen shot below):
GAAAGGTCGTGA
which is a complement of the FlyBase sequence.
The strand information is important for the analysis that I am doing so I would like to understand the discrepancy and ensure that the FlyBase sequence is indeed on the + strand. FYI, the HUMAN fasta download from biomart from ensembl and the UCSC sequence are identical.
Any help would be appreciated!
you have linked an image that seems to be in the trash