Is anyone aware of a list of vendors that have designed exome (or other 'large' scale) capture or amplification reagents? I'm mostly interested in human exomes or other large panels (say >1000 genes). The ones I'm aware of come from:
- NimbleGen (SeqCap EZ)
- Agilent (SureSelect)
- LifeTechnologies (AmpliSeq)
- Illumina (TruSeq Exome / Nextera Rapid Capture)
- Baylor College of Medicine (VCRome, synthesize by NimbleGen)
Am I missing any? What about open source initiatives where the design is published and its up to you to get the reagent synthesized
I'm particularly interested in the designs of these reagents. How do they differ in their targeted regions and performance for successfully enriching these regions for sequencing. Any recent publications that systematically compare these approaches?
Here is one publication:
A comparative analysis of exome capture (2011)
Also the design need not be limited to the 'exome' and could be any large collection of regions that is significantly more focused than the whole genome. i.e. the 'interesting-ome', by some definition. The way we define 'interesting' is the bioinformatic challenge. If one wants to sequence less than say ~2.5% (~75 MB) of the human genome and focus that target space on the most functionally relevant regions, how is that currently being defined?
Of course there is not one right answer, and it obviously depends on the biology you are conducting. I'm most interested in human disease but I'm curious to know about creative strategies for any area of human genomics.
