**730**wrote:

Hi friends

If in a RNA-seq study we have just one sample per condition,

what should we do for finding those genes which expressed differentially? (i mean those significant)

How much it'll be reliable to use one sample in DE study?

Last question, Which statistical test is proper for finding P-Value in this situation (1 sample)?

Tnx e laat ,

**89k**• written 4.8 years ago by Whoknows •

**730**

I like Devon's answer, "There is no valid statistical test for your case, at least unless you want to assume no biological variance." And in case you decide to assume no biological variance, I would add: There is no valid statistical test for your case. Nonetheless, sometimes one has to work with the data at hand. Have a look at the edgeR users guide, specifically the section titled: "What to do if you have no replicates."

6.8kYou can maybe (I strongly emphasize "maybe". I do not recommend this) try to get a common biological variance by only looking at the subset of your data that shouldn't be DE, IE. house keeping genes. But how you define house keeping genes is another whole set of problems.

Like Devon said, you need more replicates.

15k