NextSeq Error distribution?
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9.8 years ago
Yannick Wurm ★ 2.5k

The use of 2-colors on NextSeq rather than four (HiSeq + MiSeq) is likely to affect error distribution. So does anyone with good NextSeq data know the extent to which this is the case? In particular, how does this affect de novo assembly of genomes and/or transcriptomes? Would be great if someone with experience could share it :)

sequencing Assembly • 1.9k views
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