Question: (Closed) What is Index Adaptor and what is the differences between it and the regular adapter?
1
gravatar for M K
5.3 years ago by
M K470
United States
M K470 wrote:

What is Index Adapter and what is the differences between it and the regular adapter?

sequencing rna-seq next-gen • 3.4k views
ADD COMMENTlink written 5.3 years ago by M K470

Have you bothered to read anything describing how Illumina sequencing works?

ADD REPLYlink written 5.3 years ago by Devon Ryan92k

Yes, I read about it , but I still confused and why they used it.

ADD REPLYlink written 5.3 years ago by M K470

Then you need to either read more or at the very least explain what exactly you find to be confusing.

ADD REPLYlink written 5.3 years ago by Devon Ryan92k

I found two index adapters for illumina one contains 63 nt and the other on contains 64 nt and there are 6 N nt in the middle and the one that I received from sequencing center is 64 so how I can know which one is right 63 nt or 64 nt. BTW, my RNA-seq data for human and mouse.

 

ADD REPLYlink written 5.3 years ago by M K470

Read up on multiplexing.

ADD REPLYlink written 5.3 years ago by Devon Ryan92k

Do you have a specific article or note about that?

ADD REPLYlink written 5.3 years ago by M K470

Hello M K!

We believe that this post is does not fit the main topic of this site.

Please read the fine manual.

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

If you disagree please tell us why in a reply below, we'll be happy to talk about it.

Cheers!

ADD REPLYlink written 5.3 years ago by Michael Dondrup46k
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