I am very fresh in the RNA Seq data analysis area and I have a question regarding the differential gene expression analysis. I have come up with an idea to perform differential gene expression analysis by using RPKM and/or expression values from RNA Seq Data by considering all the RPKM and/or expression value outputs for each gene in separate datasets and consider each value as one replicate. I have one sample from a drug-resistant cell line and one sample from a drug-sensitive cell line. I have usually more than one RPKM values for one gene at a sample. I was thinking to consider the RPKM value for each gene as a replicate and continue with the statistical analysis and fold change calculation. Could that be possible or logical? I appreciate your help. Thank you in advance.