UTR region misassembly ?
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6.7 years ago
ascendo.u ▴ 10

Dear folks,

Helloo!

During my RNA-seq assembly, I found many misassemble or SNP or indel in UTR region.

I used SOAPdenovo-trans, Trinity and others.

They always generated lots of redundancy.

And this redundancy generated from UTR regions even though the gene body have well consensus sequence.

Cause of this UTR difference, I think they generated a lot of redundancy.

Do you have any idea of this happening?

I think the coverage of UTR is lower than gene body.

Please give me some reference.

 

Thank you.

 

 

 

UTR RNA-Seq Assembly transcriptome misassembly • 1.5k views
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did you visualize your assembly, how does it look like?

may be the case of differential expression of isoforms, but I am not sure.

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