Question: UTR region misassembly ?
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gravatar for ascendo.u
5.2 years ago by
ascendo.u10
United States
ascendo.u10 wrote:

Dear folks,

Helloo!

During my RNA-seq assembly, I found many misassemble or SNP or indel in UTR region.

I used SOAPdenovo-trans, Trinity and others.

They always generated lots of redundancy.

And this redundancy generated from UTR regions even though the gene body have well consensus sequence.

Cause of this UTR difference, I think they generated a lot of redundancy.

Do you have any idea of this happening?

I think the coverage of UTR is lower than gene body.

Please give me some reference.

 

Thank you.

 

 

 

ADD COMMENTlink modified 5.1 years ago by Biostar ♦♦ 20 • written 5.2 years ago by ascendo.u10

did you visualize your assembly, how does it look like?

may be the case of differential expression of isoforms, but I am not sure.

ADD REPLYlink written 5.1 years ago by Manvendra Singh2.1k
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