Entering edit mode
9.6 years ago
ascendo.u
▴
10
Dear folks,
Helloo!
During my RNA-seq assembly, I found many misassemble or SNP or indel in UTR region.
I used SOAPdenovo-trans, Trinity and others.
They always generated lots of redundancy.
And this redundancy generated from UTR regions even though the gene body have well consensus sequence.
Cause of this UTR difference, I think they generated a lot of redundancy.
Do you have any idea of this happening?
I think the coverage of UTR is lower than gene body.
Please give me some reference.
Thank you.
did you visualize your assembly, how does it look like?
may be the case of differential expression of isoforms, but I am not sure.