I have a mouse model with a specific pathology (7 mice total). All 7 mice have gene expression data (Agilent). I would like to identify human patients who resemble the most like this mouse model in terms of gene expression. I have several human samples with gene expression (Illumina). I have two questions:
1) How can I generate a "general gene signature" for the murine model with 7 mice?
2) How can I test each human sample with this mouse signature? Importantly, how can I establish a threshold? Moreover, would different platforms cause problem?
I know that there must be some good tools out there (e.g. Bioconductor, Broad Institute...), but I am new with gene expression. Any suggestion would be greatly appreciated.