Hi Dear All
I have a dataset (about 60m reads paired or 120m together~ 14.5Gb each file). at the first I run trinity (latest version) on my dataset with below code on my pc with 16G Ram, 8 Core CPU :
Trinity --seqType fq --JM 10G --left reads_1.fq --right reads_2.fq --CPU 8
the run failed (because RAM deficiency).
after search I found that i have to set CPU with butterfly dependency RAM (bflyHeapSpaceMax) then i change my code as follow and the all of process were OK and trinity.fasta file was created. All of results and statistics is OK (for example mapped back reads to trinity.fasta)
Trinity --seqType fq --bflyHeapSpaceMax 4G --JM 10G --left reads_1.fq --right reads_2.fq --CPU 4
and now i am really confused. because I thought that i should to run my dataset on a server with 100G RAm and ...
so please help me about this situation. is it my results ok?????
Please guide me.