Assemble PCR Product Sanger Sequences
1
0
Entering edit mode
7.0 years ago
pld 5.0k

I have a set of Sanger sequences generated from PCR products for a gene. Each component covers a specific part of the gene with the sum of the PCR products covering the whole gene. I'd like to reconstruct the gene sequence from each of these products, what is the best approach for doing this?

sequencing sanger pcr • 2.7k views
ADD COMMENT
0
Entering edit mode

I use to do it by AutoAssembler which is on Mac but we got that along with ABI 3730 sequencer. I am not sure if its available freely.

ADD REPLY
0
Entering edit mode
7.0 years ago
Manvendra Singh ★ 2.2k

I think DNAstar would be best for you. You can do it on Bio-edit also.

ADD COMMENT

Login before adding your answer.

Traffic: 2718 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6