I want to do read normalization using trinity, actually with looking the written command in the trinity's site, I'm confused about the allocated RAM to Jellyfish and coverage. It says:
TRINITY_RNASEQ_ROOT/util/insilico_read_normalization.pl --seqType fq --JM 100G --max_cov 30 --left left.fq --right right.fq \--pairs_together --PARALLEL_STATS --CPU 10.
Could anybody help me to know what means "--JM 100G --max_cov 30". what I understand, 100GB RMA is allocated to Jellyfish that seems is high for computer with 64GB RAM. please let me know the exact command for read normalization in my computer (64GB RAM). Thank a lot in advance