Question: about blast2go problem
0
gravatar for Kurban
6.0 years ago by
Kurban190
china/Urumqi/xinjiang academy of animal scinces
Kurban190 wrote:

hello.
i am using blast2go to annotate my sequences(which contain 30000 sequences), last night it annotated fine, but in the morning because of the network connection abnormality it is aborted. i tried again since then(tried with the .fa file with 4 sequences), but it is not working and constantly shows this "Negative CloudBlast Computation Units Balance: You have now consumend all CloudBlast computation units available for your subscription." why it does not working?

blast2go • 3.0k views
ADD COMMENTlink modified 5.2 years ago by Stefan30 • written 6.0 years ago by Kurban190

This clearly indicates that you have used the free quota allocated to you. You may need to pay for Blast2go PRO.

ADD REPLYlink written 6.0 years ago by geek_y11k
0
gravatar for Kurban
6.0 years ago by
Kurban190
china/Urumqi/xinjiang academy of animal scinces
Kurban190 wrote:

thanks @ Geek_y,

then i can use blast (notcloudblast)to finish my job right? if i could how long time does it take to blast around 30000 sequences?

ADD COMMENTlink written 6.0 years ago by Kurban190
0
gravatar for Stefan
5.2 years ago by
Stefan30
Spain
Stefan30 wrote:

From within Blast2GO you have various options to get your Blast+ (NCBI Blast) job done:

  • CloudBlast (paid Blast2GO PRO Community Resource Cloud System)
  • NCBI Blast (online Blast against the NCBI)
  • Local Blast (download the database of your choice and start blasting on you local PC)
  • Blast using your own Amazon Cloud Instance. 

The speed obviously depends, in case of a local blast, on your database size and number of CPUs. In case of the NCBI blast on the speed/traffic of the NCBI resources. A blastx of 30.000 sequences against the NR database could take you some 10 days at the NCBI, easily.  

Tip: Make sure you start the InterProScan at the same time since this step also takes quite a while. They can be done in parallel very nicely in Blast2GO. 

Once you have these results the functional annotation process should be relatively quick.

 

 

ADD COMMENTlink written 5.2 years ago by Stefan30

All these Blast2GO features and more are now available via OmicsBox from https://www.biobam.com/omicsbox/

ADD REPLYlink written 4 weeks ago by sgoetz12320
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