i am using blast2go to annotate my sequences(which contain 30000 sequences), last night it annotated fine, but in the morning because of the network connection abnormality it is aborted. i tried again since then(tried with the .fa file with 4 sequences), but it is not working and constantly shows this "Negative CloudBlast Computation Units Balance: You have now consumend all CloudBlast computation units available for your subscription." why it does not working?
From within Blast2GO you have various options to get your Blast+ (NCBI Blast) job done:
- CloudBlast (paid Blast2GO PRO Community Resource Cloud System)
- NCBI Blast (online Blast against the NCBI)
- Local Blast (download the database of your choice and start blasting on you local PC)
- Blast using your own Amazon Cloud Instance.
The speed obviously depends, in case of a local blast, on your database size and number of CPUs. In case of the NCBI blast on the speed/traffic of the NCBI resources. A blastx of 30.000 sequences against the NR database could take you some 10 days at the NCBI, easily.
Tip: Make sure you start the InterProScan at the same time since this step also takes quite a while. They can be done in parallel very nicely in Blast2GO.
Once you have these results the functional annotation process should be relatively quick.