Question: Tophat2 Bowtie2 inchoerent results?
gravatar for Bio_ysl
6.1 years ago by
Bio_ysl20 wrote:

I am trying to align paired-end data from Illumina (300bp x 2) to a draft genome. First of all I use Trimmomatic to remove adapters and bad quality ends. later I tried to map the reads; first with Bowtie2 alone and later with TopHat2 (which uses Bowtie2).

Bowtie2 alone mapped about 68% of the reads while Tophat about 40%, with which parameters is TopHat calling Bowtie that the alignment rate decrease instead of increasing?  Since the reads are quite long (up to 300nuc) should I modify the "--read-edit-dist" and "-N" parameters  (in this way I get more percentage of mapped reads, but I don't know if I'm getting ad alignments)?

Thank you very much


mapping rna-seq ngs tophat bowtie2 • 1.7k views
ADD COMMENTlink written 6.1 years ago by Bio_ysl20

AFAIK, tophat2 uses more strict default settings. For example tophat2 allows only 3 mismatches with default settings. There is a lot more stuff different but I can't tell from the top of my head. Therefore just try some different settings!



ADD REPLYlink written 6.1 years ago by Phil S.660
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