Question: PCR primers for CDS sequences
0
gravatar for zahraeskandari.1364
4.9 years ago by
Turkey
zahraeskandari.13640 wrote:

I want to find  genes expressed in the pancreas (GNF / Atlas organism part), with only one transcript form (transcript count = 1) and CDS length longer than 980 bp. Then I should design multiplex PCR primers for CDS sequences of these genes. I have to report PCR primers list, Gene IDs. I should use biomart twice and should use http://biocompute.bmi.ac.cn/MPprimer/

my main problem is that I dont know which option shall I select for  CDS length longer than 980 bp

Thanks inadvance

 

sequence gene • 2.5k views
ADD COMMENTlink modified 4.9 years ago by Devon Ryan92k • written 4.9 years ago by zahraeskandari.13640
0
gravatar for Devon Ryan
4.9 years ago by
Devon Ryan92k
Freiburg, Germany
Devon Ryan92k wrote:

Just get all of the CDS for genes with a single transcript and then filter the results by length with biopython/bioperl/whatever.

ADD COMMENTlink written 4.9 years ago by Devon Ryan92k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1038 users visited in the last hour