PCR primers for CDS sequences

Question

[zahraeskandari.1364]

I want to find genes expressed in the pancreas (GNF / Atlas organism part), with only one transcript form (transcript count = 1) and CDS length longer than 980 bp. Then I should design multiplex PCR primers for CDS sequences of these genes. I have to report PCR primers list, Gene IDs. I should use biomart twice and should use http://biocompute.bmi.ac.cn/MPprimer/

My main problem is that I don't know which option shall I select for CDS length longer than 980 bp

Thanks in advance

Answer 1

[Devon Ryan]

Just get all of the CDS for genes with a single transcript and then filter the results by length with biopython/bioperl/whatever.