Question: PCR primers for CDS sequences
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zahraeskandari.1364 • 0 wrote:
I want to find genes expressed in the pancreas (GNF / Atlas organism part), with only one transcript form (transcript count = 1) and CDS length longer than 980 bp. Then I should design multiplex PCR primers for CDS sequences of these genes. I have to report PCR primers list, Gene IDs. I should use biomart twice and should use http://biocompute.bmi.ac.cn/MPprimer/
my main problem is that I dont know which option shall I select for CDS length longer than 980 bp
Thanks inadvance
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modified 5.8 years ago
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Devon Ryan ♦ 97k
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5.8 years ago by
zahraeskandari.1364 • 0
