Question: Help with finding tutors for GO enrichment analysis, R, Cytoscape, Python, Linux, Perl, Octave and/or MATLAB for yeast microarray analysis, NGS and constructing gene interaction networks needed
gravatar for david.ballester
5.8 years ago by
david.ballester20 wrote:

I forward this message, may be someone could give a hand?



Student at University of Arkansas at Little Rock


I am a visually impaired bioinformatics graduate student using microarray data for my master’s thesis aimed at deciphering the mechanism by which the yeast wild type can suppress the rise of free reactive oxygen species (ROS) induced by caloric restriction (CR) thus extending lifespan but the Atg15 and Erg6 knockout mutant cannot.

Since my remaining vision is very limited I need very high magnification. But that makes my visual field very small. Therefore I need somebody to teach me how to use these programming environments, especially for microarray analysis, next generation sequencing and constructing gene and pathway interaction networks. This is very difficult for me to figure out without assistance because Zoomtext, my magnification and text to speech software, on which I am depending because I am almost blind, has problems reading out aloud many programming related websites to me. And even those websites it can read, it can only read sequentially from left to right and then from top to bottom. Unfortunately, this way of acquiring, finding, selecting and processing new information and answering questions is too tiresome, exhausting, ineffective and especially way too time consuming for graduating with a PhD in bioinformatics before my funding runs out despite being severely limited by my visual disability. I would also need help with writing a good literature review and applying the described techniques to my own yeast Affymetrix microarray dataset because I cannot see well enough to find all relevant publications on my own.

Some examples for specific tasks I urgently need help with are: 

1. GO enrichment analysis, e.g. learning how to use the TopGo R package to perform statistical analysis on GO enrichments. 

2. Analyzing and comparing the three publically available microarray datasets that can be accessed at:

3. Learning how to use the Affymetrix microarray analysis software for the Yeast 2 and Yeast S98 chip, which can be found at 

Since I am legally blind the rehab agency is giving me money to pay tutors for this purpose. Could you please help me getting in touch regarding this with anybody, who could potentially be interested in teaching me one on one thus saving me time for acquiring new information and skills, which I need to finish my thesis on time, so that I can remain eligible for funding to continue in my bioinformatics PhD program despite being almost blind? The tutoring can be done remotely via TeamViewer 5 and Skype. Hence, it does not matter where my tutors are physically located. Currently I have tutors in Croatia and UK. But since they both work full time jobs while working on their PhD dissertation they only have very limited time to teach me online. Could you therefore please forward this request for help to anybody, who could potentially be interested or, who could connect me to somebody, who might be, because my graduation and career depend on it? Who else would you recommend me to contact regarding this? Where else could I post this because I am in urgent need for help? 

Could you please contact me directly via email at and/or Skype at tfh002 and/or cell phone at (318) 243 3940 because my text to speech software has problems to read out this website aloud to me? 

I thank you very much in advance for your thoughts, ideas, suggestions, recommendations, advice, time, help, efforts and support. 

With very warm regards,

Thomas Hahn

microarray analysis ngs • 2.4k views
ADD COMMENTlink modified 5.6 years ago by Biostar ♦♦ 20 • written 5.8 years ago by david.ballester20

Thomas posted this same ad a month ago, and two months ago. If he wants to work with computers, he has to figure out how to use webpages instead of begging we do his homework.

ADD REPLYlink written 5.6 years ago by karl.stamm3.8k
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