Hi Biostar Experts,
I have to deal with RIP-Seq data.
I understand RIP-Seq in a way that the RBP bound the transcript and then the whole transcript is pulled down and sequenced. So there is no RNAase that eats away RNA sequence, as by CHIP-Seq.
If I'm right, then I don't get the point of doing peak-calling for RIP-Seq :/
So does RIP-Seq really produce peaks ?
Thanks for your help.