Question: Is it normal to find mRNA traces in small RNA-Seq ?
1
gravatar for Nicolas Rosewick
5.3 years ago by
Belgium, Brussels
Nicolas Rosewick8.8k wrote:

Hi,

I've a bunch of small RNA-Seq samples that I aligned using bowtie. Surprisingly I found some traces of mRNA expression in the alignment (reads aligning on mRNA exons). especially mRNA highly expressed (e.g. MYC). Is it normal ? I use Illumina TruSeq small RNA kit without gel excision. RNA 3' adapter is specifically modified to target microRNAs and other small RNAs that have a 3' hydroxyl group resulting from enzymatic cleavage by Dicer or other RNA processing enzymes. Thus I do not expect mRNA traces in my data ... 

Anyone experiences such thing ?

 

Thanks

smallrna-seq mrna • 1.5k views
ADD COMMENTlink written 5.3 years ago by Nicolas Rosewick8.8k
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