Question: Cutadapt 5' adapter
0
gravatar for ashwini
5.2 years ago by
ashwini100
India
ashwini100 wrote:

Hi,

I am using cutadapt to trim small RNA reads.

The following is the command used-

cutadapt -a TGGAATTCTCGGGTGCCAAGG -g TTCAGAGTTCTACAGTCCGACGATC -m 15 -M 51 --untrimmed-output=FILE_NAME IN.fastq > OUT.fastq

I have changed "U" in the actual adapter to "T" while using with -g option (RA5) and have found few reads with this adapter. Am I right in doing this?

The 5'  and 3' adapters are ( http://supportres.illumina.com/documents/documentation/chemistry_documentation/experiment-design/illumina-customer-sequence-letter.pdf )

RNA 5’ Adapter (RA5), part # 15013205
5’ GUUCAGAGUUCUACAGUCCGACGAUC

RNA 3’ Adapter (RA3), part # 15013207
5’ TGGAATTCTCGGGTGCCAAGG

 

 

 

cutadapt 5' adapter smallrna • 2.2k views
ADD COMMENTlink modified 5.2 years ago by Fabio Marroni2.5k • written 5.2 years ago by ashwini100
0
gravatar for Fabio Marroni
5.2 years ago by
Fabio Marroni2.5k
Italy
Fabio Marroni2.5k wrote:

Yes, you are right.

Since you are actually sequencing cDNA, you will have T and not U.

 

 

ADD COMMENTlink written 5.2 years ago by Fabio Marroni2.5k
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